Enhancement of polynucleotide hybridization
US5512436A · kind A · utility
21Cited by
2References
14Claims
0Family size
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Inventor
Key dates
| Filing date | Sep 2, 1993 |
| Grant date | Apr 30, 1996 |
| Priority date | — |
| Expiry date | Sep 2, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6832
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Hybridization buffers, for hybridizing complementary polynucleotides, contain polyvinyl alcohol (MW 1000-20000) and/or polystyrene sulphonic acid (e.g. MW 60000-80000) as a rate enhancer, generally at a concentration of 1-10%. Dextran sulphate, polyethylene glycol and cationic detergents may be additionally present. The method is useful when one of the two complementary polynucleotides is immobilised, or is in in situ hybridizations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.