Oligonucleotide-linked magnetic particles and uses thereof

US5512439A · kind A · utility

Assignee

• Dynal AS · NO

Inventors

• Erik Hornes · Oslo, NO
• Lars Korsnes · Oslo, NO

Key dates

• Filing date: Jul 6, 1994
• Grant date: Apr 30, 1996
• Expiry date: Jul 6, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6816
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Monodisperse, superparamagnetic particles carrying a plurality of molecules of an oligonucleotide are disclosed and may be used inter alia for sequencing single-stranded nucleic acids. The oligonucleotide may be covalently attached or affinity bonded to the particles either by their 3' or 5' termini. The particles have a specific gravity in the range 1.1-1.8 and are 1-10 microns in diameter. A kit for the isolation or processing of target nucleic acid is also disclosed.