Method for the improved production and recovery of poly-.beta.-hydroxybutyrate from transformed Escherichia coli
US5512456A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 29, 1992 |
| Grant date | Apr 30, 1996 |
| Priority date | — |
| Expiry date | May 29, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/625
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Poly-.beta.-hydroxybutyrate is produced by providing a culture of Escherichia coli bacterial host cells transformed by a DNA sequence coding for the biosynthetic pathway of poly-.beta.-hydroxybutyrate and a DNA sequence coding for the lysozyme gene; growing the culture and obtaining expression of the poly-.beta.-hydroxybutyrate biosynthetic pathway and the lysozyme gene in each Escherichia coli bacterial host cell; lysing the Escherichia coli bacterial host cells and collecting the poly-.beta.-hydroxybutyrate. An Escherichia coli HMS174(p4A BstB!, pLysS) deposited with the American Type Culture Collection under Accession No. 69001, comprising a plasmid containing a biosynthetic pathway coding for poly-.beta.-hydroxybutyrate and a plasmid containing a lysozyme gene is disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.