Process for purifying an .alpha.-D-galactosidase isozyme from Coffea beans
US5512471A · kind A · utility
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19Claims
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Key dates
| Filing date | Dec 23, 1992 |
| Grant date | Apr 30, 1996 |
| Priority date | — |
| Expiry date | Dec 23, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A Coffea canephora-D-galactosidase isozyme is purified by extracting a supernatant from Coffea beans containing the isozyme, extracting tannin from the supernatant, and isolating the isozyme. Preferrably, the supernatant is extracted by exposing the supernatant to insoluble polyvinylpolypyrrolidone in an amount sufficient to remove the tannin from the supernatant by forming hydrogen bonds with the tannin.
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