Bicarbonate assay and phosphoenolpyruvate carboxylase enzyme from hyphomicrobium
US5514538A · kind A · utility
5Cited by
2References
28Claims
0Family size
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Key dates
| Filing date | Mar 30, 1993 |
| Grant date | May 7, 1996 |
| Priority date | — |
| Expiry date | Mar 30, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Phosphoenolpyruvate carboxylase (PEPC) characterized by a specific activity of at least 40 units/mg; and/or by no more than 50% loss of activity after 60 days in 167 mM Tricine/HCl buffer, pH 8.0, and 15 mM MgCl.sub.2 ; and/or, at pH 6.5, an activity of at least 40% relative to the activity at optimum pH is disclosed. Such enzymatic activity can be obtained from serine pathway methylotrophs such as Hyphomicorbium, and is very suitable for use in measuring bicarbonate in biological samples.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.