Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5514545A · kind A · utility
119Cited by
2References
6Claims
0Family size
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Key dates
| Filing date | Jul 26, 1993 |
| Grant date | May 7, 1996 |
| Priority date | — |
| Expiry date | Jul 26, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for characterizing cells at the molecular level by amplifying RNA from selected single cells by microinjecting primer, nucleotides and enzyme into acutely dissociated cells to produce amplified antisense RNA; reamplifying the amplified antisense RNA (aRNA) produced by using random hexanucleotides to prime cDNA synthesis from aRNA, and then detecting messages in the amplified RNA. The invention is useful for characterization of cell identity or physiological state.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.