Stem-loop oligonucleotides containing parallel and antiparallel binding domains
US5514546A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 1, 1993 |
| Grant date | May 7, 1996 |
| Priority date | — |
| Expiry date | Sep 1, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6839
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides stem-loop oligonucleotides containing a double-stranded stem domain of at least about 2 base pairs and a single-stranded loop domain. The loop domains of the present oligonucleotides include at least one parallel binding (P) domain separated by at least about 3 nucleotides from a corresponding anti-parallel binding (AP) domain. Each P and corresponding AP domain of the present oligonucleotides can bind detectably to one strand of a defined nucleic acid target wherein the P domain binds in a parallel manner to the target and the corresponding AP domain binds in an anti-parallel manner to the target. The present stem-loop oligonucleotides can bind to both single-stranded and double-stranded target nucleic acids. The present invention also provides methods of using these oligonucleotides as well as kits and pharmaceutical compositions containing these oligonucleotides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.