Method for in vivo selection of ligand-binding proteins
US5514548A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 17, 1994 |
| Grant date | May 7, 1996 |
| Priority date | — |
| Expiry date | Feb 17, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The application discloses a method, and kit, which enables the selection of proteins or other oligo- or polypeptides (collectively, ligand binding proteins, LBPs) which bind with high affinity to a target ligand or receptor. The method relies on the display of the LBP of interest on the surface of replicable genetic packages (RGPs) which are modified so as to be non-infective. Infectivity is restored to those RGPs displaying LBPs with high affinity for a target ligand or receptor by an infectivity mediating complex. The infectivity mediating complex comprises the ligand or receptor covalently linked to a polypeptide which brings about interaction between the RGP and a host cell. It is envisaged that the LBP of interest will belong to a genetically diverse collection of similar substances. The method therefore allows selection of substances with high affinity for target, from within a large collection of variants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.