Nucleic acid test article and its use to detect a predetermined nucleic acid
US5514550A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 2, 1994 |
| Grant date | May 7, 1996 |
| Priority date | — |
| Expiry date | Sep 2, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6876
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A nucleic acid test article can be used to detect a targeted nucleic acid found in a specimen. The test article includes a substrate having two opposing surfaces and a water-insoluble nucleic acid probe attached in a distinct zone of one of the surfaces. The probe is prepared from a water-insoluble particle to which is covalently attached an oligonucleotide which is complementary to the targeted nucleic acid. Substantially none of the probe is embedded within the surface of the substrate. Particularly useful test articles have a multiplicity of water-insoluble probes located in distinct zones on one of the substrate surfaces. These probes are useful for the detection of a multiplicity of targeted nucleic acids, particularly after amplification by polymerase chain reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.