Patent · US Expired

Yeast processing system

US5514585A · kind A · utility

2Cited by

0References

7Claims

0Family size

Assignee

Novo Nordisk A/S · DK

Inventors

S.o slashed.ren E. Bj.o slashed.rn · Avenue Madeleine, DK
Kjeld Norris · Copenhagen, DK
Fanny Norris · Hellerup, DK

Key dates

Filing date	Mar 2, 1995
Grant date	May 7, 1996
Priority date	—
Expiry date	Mar 2, 2015

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/75
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A polypeptide for production in yeast comprises a fusion of a signal peptide, a leader peptide and a heterologous protein or polypeptide. The polypeptide is modified in its amino acid sequence adjacent to a yeast processing site positioned between the C-terminal end of the leader peptide and the N-terminal end of the heterologous protein so as to provide a presentation of the processing site which makes it accessible to proteolytic cleavage. Such a presentation is provided by adding one or more amino acids (at least one of which is negatively charged) to either the C-terminal end of the leader or the N-terminal end of the protein, or both. The heterologous protein may, for instance, be aprotinin or insulin precursor or an analogue thereof.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.