Primer directed nucleic acid amplification including the addition of specific template DNA inactivating enzyme
US5516292A · kind A · utility
13Cited by
2References
6Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 2, 1991 |
| Grant date | May 14, 1996 |
| Priority date | — |
| Expiry date | May 2, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for the enzymatic reduction and/or elimination of contaminant DNA from enzymatic amplification procedures are disclosed. These methods include the use of restriction endonucleases, DNAases and exonucleases which act upon contaminant DNA in polymerase chain reaction mixtures such that contaminant DNA is not amplified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.