Method for producing the SSPI restriction endonuclease and methylase
US5516678A · kind A · utility
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7Claims
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Key dates
| Filing date | Oct 6, 1994 |
| Grant date | May 14, 1996 |
| Priority date | — |
| Expiry date | Oct 6, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for cloning and producing the SspI restriction endonuclease by 1) introducing the restriction endonuclease gene from Sphaerotilus species into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the SspI restriction endonuclease, and 3) purifying the SspI restriction endonuclease from the fermented host which contains the vector encoding and expressing the SspI restriction endonuclease activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.