Dye forming composition and detection of hydrogen peroxide therewith
US5518891A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 25, 1993 |
| Grant date | May 21, 1996 |
| Priority date | — |
| Expiry date | Aug 25, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/904
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A color-forming coupler is provided which can be used in test compositions, as well as methods and apparatus for detection of hydrogen peroxide. The color-forming coupler compounds react with a hydrazone or an aminoantypyrine in the presence of peroxidase and hydrogen peroxide to produce a darkly colored compound. In the presence of peroxidase and hydrogen peroxide, the couplers of the present invention react with a hydrazone or an aminoantipyrine to produce a deeply colored dye, which can be used to give a visual indication of the analyte present in the sample. These couplers can be used in diagnostic tests in which the analyte is converted to hydrogen peroxide. The color-forming couplers are N,N-disubstituted anilines of the formula shown in FIG. 1 , wherein R.sup.1 and/or R.sup.2 =H, C.sub.1 -C.sub.9 alkyl, C.sub.1 -C.sub.9 -C.sub.9 alkoxy, NR.sup.3 R.sup.4 (where R.sup.3 and/or R.sup.4 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroryl), F, Cl, Br, I, COOR.sup.5 (where R.sup.5 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteroaryl), CN, CONR.sup.6 R.sup.7 (where R.sup.6 and/or R.sup.7 =H, C.sub.1 -C.sub.9 alkyl, aryl, or heteraryl), aryl, aryloxy, heteroaryl, heteroaryloxy; and R.sup.11, …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.