Urokinase-type plasminogen activator receptor antibodies
US5519120A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 17, 1993 |
| Grant date | May 21, 1996 |
| Priority date | — |
| Expiry date | Jun 17, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/9723
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A monoclonal or polyclonal antibody directed against urokinase plasminogen activator receptor (u-PAR), or a subsequence, analogue or glycosylation variant thereof. Antibodies are disclosed which react with free u-or with complexes between u-PA and u-and which are capable of 1) catching u-in ELISA, or 2) detecting u-PAR, e.g. in blotting, or 3) in radioimmunoprecipitation assay precipitate purified u-in intact or fragment form, or 4) is useful for immunohistochemical detection of u-PAR, e.g. in immunostaining of cancer cells, such as in tissue sections at the invasive front, or 5) inhibits the binding of pro-u-PA and active u-PA and thereby inhibits cell surface plasminogen activation. Methods are disclosed 1) for detecting or quantifying u-PAR, 2) for targeting a diagnostic to a cell containing a u-on the surface, 3) for preventing or counteracting proteolytic activity in a mammal. Methods for for selecting a substance suitable for inhibiting u-PA/u-interaction, for preventing or counteracting localized proteolytical activity in a mammal, for inhibiting the invasion and/or metastasis comprise the use of the antibodies and of nude mice inoculated with human cancer cells which are kn…
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