Process for preparing boron, aluminum, and phosphorus silicates for purification of DNA
US5520899A · kind A · utility
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Key dates
| Filing date | Apr 20, 1995 |
| Grant date | May 28, 1996 |
| Priority date | — |
| Expiry date | Apr 20, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldMaterials, metallurgy
- WIPO sectorChemistry
Abstract
A process for preparing borosilicate, aluminosilicate, or phosphosilicate comprising preparing a mixture of SiCl.sub.4 with a specific molar ratio of BCl.sub.3, AlCl.sub.3, or PCl.sub.3 ; cooling said mixture to zero degrees; and adding water to the reaction mixture until the evolution of gas is complete. These silicates are useful for purifying DNA wherein the DNA is bound in water or low salt buffers (i.e., in the absence of a chaotropic agent or alcohol).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.