Secretion sequence for the production of a heterologous protein in yeast
US5521086A · kind A · utility
34Cited by
4References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 16, 1993 |
| Grant date | May 28, 1996 |
| Priority date | — |
| Expiry date | Sep 16, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention generally features a novel recombinant DNA molecule useful for producing a heterologous protein, e.g., insulin-like growth factor-I, in yeast. The DNA molecule includes the following sequences joined in frame and in the following 5' to 3' progression: a sequence encoding a signal peptide of a yeast secreted protein; a sequence encoding at least 5 amino acids of the N-terminal region of a mature acid phosphatase protein, a sequence encoding a heterologous protein. Preferably the DNA molecule also encodes a spacer element that includes a proteolytic cleavage site.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.