Method for the directional cloning of DNA
US5523221A · kind A · utility
17Cited by
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16Claims
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Key dates
| Filing date | Jun 16, 1993 |
| Grant date | Jun 4, 1996 |
| Priority date | — |
| Expiry date | Jun 16, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for directionally cloning an insert DNA fragment into a target sequence using differential phosphorylation is disclosed, Monophosphorylated PCR fragments are directionally cloned into a monophosphorylated plasmid, Methods for directionally cloning non-PCR fragments into target DNA sequences are also discussed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.