Methods of synthesizing oligonucleotides with random codons
US5523388A · kind A · utility
69Cited by
4References
20Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Feb 27, 1995 |
| Grant date | Jun 4, 1996 |
| Priority date | — |
| Expiry date | Feb 27, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention provides a method of synthesizing oligonucleotides having random tuplets using individual monomers. The steps consist of: (1) sequentially coupling monomers on separate supports to form at least two different tuplets, the coupling is performed in separate reaction vessels; (2) mixing the supports from the reaction vessels; (3) dividing the mixed supports into two or more separate reaction vessels; and (4) repeating steps (1) through (3) one or more times in the reaction vessels of step (3), wherein the last step ends at step (2). Additionally, the oligonucleotides can be cleaved from the supports.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.