Purification of DNA on aluminum silicates, and phosphosilicates
US5523392A · kind A · utility
11Cited by
5References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 11, 1994 |
| Grant date | Jun 4, 1996 |
| Priority date | — |
| Expiry date | Mar 11, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldMaterials, metallurgy
- WIPO sectorChemistry
Abstract
A process for purifying DNA comprising binding the DNA to a silicate compound in the presence of water, low salt buffer, or physiological buffer and eluting in the bound DNA by heating in a low salt buffer, a physiological buffer, or water. The silicate compound is prepared by reacting SiCl.sub.4 with a molar percentage of BCl.sub.3 of about 33.5%, 55.6%, or 83.3%, with a molar percentage of PCl.sub.3 of about 50.0%-83.3%, or with a molar percentage of AlCl.sub.3 of about 9.1%; cooling the reaction mixture; adding water until the evolution of gas is complete; and recovering the compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.