Thermal cycler including a temperature gradient block
US5525300A · kind A · utility
240Cited by
12References
8Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 20, 1993 |
| Grant date | Jun 11, 1996 |
| Priority date | — |
| Expiry date | Oct 20, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method in which a temperature gradient is generated across a "gradient" block, and an apparatus comprising a block across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells on the gradient block can be simultaneously run at temperatures which differ only slightly, thereby permitting an optimum temperature for the reaction to be quickly identified. In a preferred embodiment the gradient block is integrated into a thermal cycler used for nucleic acid amplification reactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.