Method for production and purification or recombinant Apolipoprotein E from bacteria
US5525472A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 3, 1994 |
| Grant date | Jun 11, 1996 |
| Priority date | — |
| Expiry date | Nov 3, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/803
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Applicants describe methods for purifying human ApoE or analog thereof from recombinant bacterial cells with minimal protein aggregation and degradation during the purification process. The invention involves the addition of neutralized fatty acids to the medium during cell disruption and the use of a non-ionic detergent during the purification process. Additionally applicants describe a method for increasing the production of ApoE or analog thereof in a bacterial host by adding to the culture medium neutralized fatty acids, fatty acid precursors, triglycerides, triglyceride precursors or acetate. Pharmaceutical and diagnostic uses of the ApoE analog are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.