Covalent dimer of kit ligand
US5525708A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 28, 1994 |
| Grant date | Jun 11, 1996 |
| Priority date | — |
| Expiry date | Mar 28, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/475
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A modified form of KL, the ligand for the c-Kit proto-oncogene, has been prepared wherein the protein is stabilized by an intermolecular covalent linkage. The protein can be prepared by expression of a recombinant protein which is dissolved in denaturant and refolded under conditions resulting in a disulfide linked dimer. Examples demonstrate the purification and characterization of this disulfide-linked cysteine dimer kit ligand (KL-CD) which contains at least one intermolecular disulfide bond and has at least ten-fold greater activity in promoting cell proliferation than native, non-covalently linked KL, as measured in in vitro assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.