Electrochemical denaturation of double-stranded nucleic acid
US5527670A · kind A · utility
218Cited by
2References
30Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Aug 9, 1994 |
| Grant date | Jun 18, 1996 |
| Priority date | — |
| Expiry date | Aug 9, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is described for denaturing native double-stranded nucleic acid material into its individual strands in an electrochemical cell. The process disclosed is an electrical treatment of the nucleic acid with a voltage applied to the nucleic acid material by an electrode. The process may also employ a promotor compound such as methyl viologen to speed denaturation. The process may be used in the detection of nucleic acid by hybridizing with a labelled probe or in the amplification of DNA by a polymerase chain reaction or ligase chain reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.