Delta-endotoxin expression in pseudomonas fluorescens
US5527883A · kind A · utility
26Cited by
11References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 6, 1994 |
| Grant date | Jun 18, 1996 |
| Priority date | — |
| Expiry date | May 6, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Bacillus thuringiensis endotoxin expression in Pseudomonads can be improved by modifying the gene encoding the Bacillus thuringiensis endotoxin. Chimeric genes are created by replacing the segment of the Bacillus thuringiensis gene encoding a native protoxin with a segment encoding a different protoxin. Exemplified herein is the cryIF/cryI(b) chimera wherein the native cryIF protoxin segment has been substituted by the cryIA(b) protoxin segment, to yield improved expression of the cryIF toxin in Pseudomonads. The invention also concerns novel genes and plasmids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.