DNA detector and DNA detection method
US5529679A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 7, 1994 |
| Grant date | Jun 25, 1996 |
| Priority date | — |
| Expiry date | Nov 7, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/00237
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An electrophoresis apparatus includes a plurality of first capillaries containing a separation medium, such as a gel, and an optical cell in which one end of each of the first capillaries is disposed. Samples labeled with fluorophores are introduced into the first capillaries, and an electric field is applied to the first capillaries to cause the samples to migrate through the first capillaries into the optical cell. A light source excites the fluorophores with light when the samples are in the optical cell, causing the fluorophores to emit fluorescence, and a photodetecting system detects the fluorescence emitted by the fluorophores. The ends of the first capillaries in the optical cell may be arranged in a straight line. The light source may include a He-Ne laser emitting light having a wavelength of 594 nm, and the fluorophores may include either sulforhodamine or a derivative of sulforhodamine. The apparatus may include a plurality of second capillaries each having one end disposed in the optical cell such that the ends of the second capillaries are separated from the ends of the first capillaries by respective gaps, or may include a single second capillary having one end dispo…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.