Analytical system or kit for phosphatase
US5529906A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 18, 1993 |
| Grant date | Jun 25, 1996 |
| Priority date | — |
| Expiry date | Nov 18, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A system or kit for analysis of phosphatase comprising a reagent I containing NADP or NADPH as a main component, and a reagent II containing components for color-development signal amplification utilizing NAD-NADH interconversion, wherein at least one of the hydrogen donors participating in the NAD-NADH interconversion is included in reagent I, but not in reagent II, which eliminates the necessity that the reagent II is further separated into two parts. This enables the preparation of reagent II as a mixture which can be readily used and which can be preserved for a long period of time without "reagent blank" occurring. Additionally, the present kit eliminates the necessity of mixing reagents I and II immediately before actual use and thus permits periodical analysis of samples with a reagent having the same components for long time periods with reliable and uniform sensitivity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.