Lactococcus promoters and signal sequences for heterologous gene expression in bacteria
US5529908A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 10, 1993 |
| Grant date | Jun 25, 1996 |
| Priority date | — |
| Expiry date | Feb 10, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
DNA sequences, derived from Lactococcus lactis subsp. lactis, are useful as promoters and promoter/secretion promoting signals for heterologous or homologous expression in Gram-positive bacteria. In another aspect, the invention relates to vectors, such as plasmids, comprising the sequences of the present invention, and to host cells transformed with such vectors. Yet additional aspects of the present invention are related to methods for producing desired heterologous or homologous peptides or proteins employing the sequences, vectors, or transformed hosts of the invention. By means of the invention, greatly improved heterologous and homologous expression and secretion may be achieved in E. coli and in Gram-positive bacteria such as B. subtilis, Lactococcus and Lactobacillus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.