Method for testing causative microorganisms of food poisioning and reagents therefor
US5529910A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 27, 1993 |
| Grant date | Jun 25, 1996 |
| Priority date | — |
| Expiry date | Sep 27, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/879
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for testing causative bacterial species of food poisoning which is characterized by using two oligonucleotide primers that hybridize to opposite strands of bacterial DNA specifically, and flank a unique region in the target DNA and amplifying the specific fragment of the bacterial DNA, comprising the steps of: PA1 (a) hybridizing the primer to specific gene sequence of bacteria in a sample, extending the hybridized primer with deoxynucleotide triphosphates (dATP, dCTP, dGTP, and dTTP), and resultantly making the double strand nucleotide; PA1 (b) where the primer extension products are cleaved into each single strand of nucleotide by certain external force such as heat, pH and so on, one single strand functioning as a template for nucleotide extension with a primer of the other strand; PA1 (c) repeating a series of cycles involving cleavage of primer extension products, primer hybridizing, extension of the hybridized primers to amplify the specific fragment of DNA, and detecting the amplified DNA fragment; and PA1 (d) as the result, determining whether or not the specific fragment is present in said sample, thereby to confirm a species of causative bacteria of food poisonin…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.