Method for producing cytoplasmic male sterility in plants and use thereof in production of hybrid seed
US5530191A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 24, 1994 |
| Grant date | Jun 25, 1996 |
| Priority date | — |
| Expiry date | Mar 24, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S47/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a cytoplasmic male sterility (CMS) system for plants, which is based on modification of the plastid genome. The CMS system comprises three transgenes: a "plastid male sterility" (pms) gene, regulated by a "nuclear male sterility" (rims) gene and a "restorer of male fertility" (rmf) gene. The pms gene is either an inactive gene encoding a toxic gene product or an actively expressed gene encoding an essential gene product. The runs gene is controlled by an anther-specific promoter, and encodes a plastid-directed polypeptide that regulates the pms gene, either by activating expression of the inactive toxic pms gene or by repressing expression of the essential pms gene, which causes plastid and cellular disablement or death in anther tissue, thereby preventing formation of viable pollen. The rmf gene encodes a gene product that prevents the rims gene from regulating the pms gene. Cytoplasmically male-sterile plants are obtained by crossing a parent line containing the pms gene with a parent line containing the nms gene. Male-fertile hybrid seed is obtained from crosses between the cytoplasmically male-sterile plants and a second parent line containing the…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.