Method and kits for determining peroxidatively active catalysts

US5532138A · kind A · utility

Assignee

• Behringwerke Aktiengesellschaft · DE

Inventors

• Sharat Singh · San Jose, US
• Arthur C. Switchenko · Palo Alto, US
• Cheng-I Lin · Cupertino, US
• Nurith Kurn · Palo Alto, US
• Edwin F. Ullman · Atherton, US

Key dates

• Filing date: Jun 21, 1994
• Grant date: Jul 2, 1996
• Expiry date: Jun 21, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/975
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions are disclosed for determining a peroxidatively active catalyst. The methods comprise the step of detecting a substance formed by the coupling reaction of (a) the product of the peroxidatively active catalyst-catalyzed oxidation of a benzidine with (b) a coupler other than benzidine. The methods have application in a wide variety of systems including assays for analytes. Also disclosed are kits for conducting methods and assays in accordance with the present invention.