Method for rendering ligase-based amplification products unamplifiable
US5532146A · kind A · utility
2Cited by
10References
16Claims
0Family size
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Key dates
| Filing date | Jun 20, 1994 |
| Grant date | Jul 2, 1996 |
| Priority date | — |
| Expiry date | Jun 20, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Methods for sterilization of ligase-based amplification products, including products of the Ligation Amplification Reaction (LAR), involving treatment of nucleic acid after amplification to render such nucleic acid incapable of serving as a template for further amplification. Both random and site-specific addition of sterilizing compounds, and more specifically, photoactivation compounds, render amplification product subsequently unamplifiable. Self-sterilizing primers are also employed allowing for a homogenous detection of ligase-based amplification product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.