Hybrid proteins between an extracytoplasmic enzyme and at least another protein, method for preparing them and also their applications

US5534223A · kind A · utility

Assignee

• COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES · FR

Inventors

• Paul Boquet · Marly-le-Roi, FR
• Jean-Claude Boulain · Palaiseau, FR
• Frederic Ducancel · Longjumeau, FR
• Daniel Gillet · Paris, FR
• Andre Menez · Voisins-le-Bretonneux, FR

Key dates

• Filing date: Jun 30, 1994
• Grant date: Jul 9, 1996
• Expiry date: Jun 30, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/975
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A kit for carrying out an immunoenzymological assay for protein, containing a diagnostic reagent consisting of a hybrid protein comprising, in order, a polypeptide P1 which is an N-terminal portion of mature alkaline phosphatase comprising from 6 to 28 of the N-terminal amino acids of said mature alkaline phosphatase, joined to a polypeptide P2, capable of interacting with an antibody or antigen joined to a polypeptide P3 which is selected from the group consisting of a) the C-terminal remaining amino acids of said mature alkaline phosphatase, b) the mature sequence of alkaline phosphatase, and c) enzymatically active fragments of b).