Glucose responsive insulin secreting .beta.-cell lines and method for producing same
US5534404A · kind A · utility
24Cited by
1References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 10, 1994 |
| Grant date | Jul 9, 1996 |
| Priority date | — |
| Expiry date | Mar 10, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/14
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of selecting cells with enhanced secretion of a secretory product is disclosed. The method comprises exposing a population of cells to a secretagogue to result in the secretion of a secretory product from the cells and selecting from the population, cells that exhibit increased amounts of intracellular free calcium when exposed to the secretagogue. The method enables the selection of correctly regulated .beta. cells that secrete appropriate amounts of insulin in response to varying glucose levels.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.