Controlled expression of recombinant proteins
US5534418A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 10, 1993 |
| Grant date | Jul 9, 1996 |
| Priority date | — |
| Expiry date | Dec 10, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods for the controlled production of recombinant proteins in cells. Cells employed in the invention method contain a gene encoding the desired recombinant protein, with transcription of the gene maintained under the control of a transcriptional control element which is activated by a ligand/receptor complex. The ligand/receptor complex is formed when a ligand (which is a hormone or/and analog thereof) is complexed with a receptor (which is a hormone receptor or functional analog thereof which has the transcription activating properties of the receptor). Receptor is produced by the expression of non-endogenous DNA which is also present in the cells used for production of recombinant protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.