Patent · US Expired

Pseudomonas fluorescens lipase

US5534427A · kind A · utility

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6Claims
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Assignee

Inventors

Key dates

Filing dateJul 31, 1992
Grant dateJul 9, 1996
Priority date
Expiry dateJul 31, 2012

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N9/20
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Nucleic acid sequences, particularly DNA sequences, coding for all or part of a lipase of Pseudomonas fluorescens, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the BamHI/HindIII insert of the plasmid pRJ-Ltac1 from Escherichia coli BL21/pRJ-Ltac1 deposited with the American Type Culture Collection, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of a lipase of Pseudomonas fluorescens, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the plasmid pRJ-Ltac1 from Escherichia coli BL21/pRJ-Ltac1 BamHI/HindIII insert of deposited with the American Type Culture Collection, and methods for producing these polypeptides.

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