Chemiluminescent 1,2-dioxetanes
US5538847A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 7, 1993 |
| Grant date | Jul 23, 1996 |
| Priority date | — |
| Expiry date | May 7, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2334/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Spiroadamantyl dioxetanes bearing an alkoxy substituent, and an aromatic substituent of phenyl or naphthyl on the dioxetane ring can be activated to chemiluminesce if the aromatic substituent bears a moiety designated OX, wherein the X is cleaved by an enzyme with which the dioxetane is permitted to come in contact with. The T.sub. 1/2 kinetics of the chemiluminescent reaction, as well as the signal intensity, or quantum yield of the chemiluminescent reaction, can be altered by selection of an electron-withdrawing or an electron-donating group Z, at positions on the aromatic substituent other than those adjacent the point of attachment to the dioxetane. Signal strength can further be enhanced by recognized chemiluminescent enhancers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.