Method of preparing nucleotide probes using a bridging complement
US5538872A · kind A · utility
7Cited by
6References
32Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 8, 1994 |
| Grant date | Jul 23, 1996 |
| Priority date | — |
| Expiry date | Sep 8, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides labeled nucleotide probes and a procedure for synthesis of the labeled nucleotide probe by a coupling or joinder of two components of the probe, namely a target recognition sequence moiety and a signal generating moiety, through the use of a bridging complement that holds the two moieties in such a position as to allow formation of a sugar-phosphate chemical linkage between the 3' end of one moiety and the 5' end of the other moiety.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.