Process for the preparation of pyruvic acid using permeabilized transformants of H. polymorha and P. pastoris which express glycolate oxidase and catalase
US5538875A · kind A · utility
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Key dates
| Filing date | Jun 22, 1995 |
| Grant date | Jul 23, 1996 |
| Priority date | — |
| Expiry date | Jun 22, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the production of pyruvic acid involving the enzymatic reaction of L-lactic acid and oxygen in an aqueous solution in the presence of catalysts glycolate oxidase ((S)-2-hydroxy-acid oxidase, EC 1.1.3.15) and catalase (EC 1.11.1.6). High yields of pyruvate at high purity can be achieved at commercially acceptable concentration without substantial product inhibition of the enzyme.
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