Method for producing secretable glycosyltransferases and other golgi processing enzymes
US5541083A · kind A · utility
5Cited by
4References
8Claims
0Family size
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Key dates
| Filing date | Mar 10, 1994 |
| Grant date | Jul 30, 1996 |
| Priority date | — |
| Expiry date | Mar 10, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/26
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.