Expression cloning of c-src SH3-domain binding proteins

US5541109A · kind A · utility

Assignee

• Rhone-Poulenc Rorer Pharmaceuticals Inc. · US

Inventors

• George H. Searfoss, III · Reading, US
• Yuri Ivashchenko · Hattersheim, DE
• Michael C. Jaye · Glenside, US
• Victoria J. South · Collegeville, US
• Stephen M. French · Nottingham, GB
• Christopher Scott Atherton Cheadle · West Chester, US
• George A. Ricca · Blue Bell, US

Key dates

• Filing date: Apr 19, 1994
• Grant date: Jul 30, 1996
• Expiry date: Apr 19, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/47
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides a unique SH3 binding domain core motif of the sequence RPLPXXP and cDNA clones encoding proteins which interact with the SH3 domain of c-src, as well as the amino acid sequences which mediate this binding. Another embodiment of this invention is a method of identifying SH3-binding proteins and elucidating the sequences which mediate binding. This method may be used as an assay to select compounds which bind to this site and which inhibit or enhance the binding of the SH3 domain.