Real-time scanning fluorescence electrophoresis apparatus for the analysis of polynucleotide fragments
US5543026A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 7, 1994 |
| Grant date | Aug 6, 1996 |
| Priority date | — |
| Expiry date | Feb 7, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44721
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to an improved real-time scanning fluorescence electrophoresis apparatus for the electrophoretic analysis of fluorescently-labeled polynucleotide fragments. The apparatus is characterized by having an electrophoresis chamber containing an electrophoretic separation medium capable of accommodating multiple electrophoresis lanes arranged in a planar array, a fluorescence detector mounted on a translatable stage, a light source for exciting fluorescent molecules, and a computer for collecting data consisting of time, location, fluorescence wavelength and fluorescent intensity information. The improvements herein disclosed include, (i) using a spectral-array detector for detecting the emission light from the fluorescently-labeled polynucleotide fragments including the simultaneous detection of multiple fluorescent labels, and, (ii) a temperature control means to control the temperature of the electrophoretic separation medium during electrophoresis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.