Injectable culture medium for maintaining viability of myoblast cells
US5543316A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Apr 20, 1994 |
| Grant date | Aug 6, 1996 |
| Priority date | — |
| Expiry date | Apr 20, 2014 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N1/10
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
An injectable grade medium for maintaining cells comprising: (a) a sugar; (b) a serum albumin; (c) a calcium salt; (d) a phosphate salt; (e) a source of iron; (f) at least one salt of a Group I element; (g) at least one salt of a Group II element; (h) at least one amino acid; and (i) at least one vitamin. Components (a) through (i) are present in the medium in amounts in order to provide an osmolality of the medium of from about 320 mOsm/kg to about 550 mOsm/kg. Such an injectable grade medium has an increased osmolality as compared with conventional media, and thus such medium may be employed in order to maintain cells over extended periods of time and for injecting such cells into a desired transplantation site of a patient, whereby such cells are transplanted into the patient, and are capable of functioning in the patient.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.