Biosensor including chemically modified enzymes

US5543326A · kind A · utility

Inventors

• Adam Heller · Austin, US
• Ioanis Katakis · San Antonio, US
• Ling Ye · Zhejiang, CN

Key dates

• Filing date: Mar 4, 1994
• Grant date: Aug 6, 1996
• Expiry date: Mar 4, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/817
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Oxidoreductases are chemically modified so as to change their net charge at a given pH. Through such modification the adsorption of the enzymes on surfaces, their retention in similarly or oppositely charged polymer membranes and their binding with similarly or oppositely charged macromolecules, particularly redox macromolecules, is controlled. The modified enzymes, of which modified recombinant glucose oxidase is an example, are useful in biosensors, e.g. for glucose; as marker enzymes of antigens or antibodies in immunosensing; and as labels of nucleotide sequences in probes, e.g. for DNA nucleotide sequences.