Patent · US Expired

Production of enzymes in seeds and their use

US5543576A · kind A · utility

42Cited by
13References
15Claims
0Family size

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Key dates

Filing dateNov 2, 1993
Grant dateAug 6, 1996
Priority date
Expiry dateNov 2, 2013

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Y301/03026
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.