Suppression of electroendosmosis during electrophoresis in gel-free polymer media by use of charged polymers
US5545302A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 5, 1995 |
| Grant date | Aug 13, 1996 |
| Priority date | — |
| Expiry date | Apr 5, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44752
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The phenomenon of electroendosmosis which occurs in electrophoretic separations and interferes with the efficiency, accuracy and reproducibility of the separations, is suppressed by the inclusion in the separation medium of a dissolved hydrophilic polymer which has been modified by amine groups covalently bonded thereto, in a controlled proportion relative to the polymer. This modified polymer suppresses electroendosmosis in both flee zone electrophoresis and molecular sieve electrophoresis. When this modified polymer is used in a capillary, the reduction which it produces in the electroendosmosis is the same as that achieved by coating the capillary wall with a neutral polymer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.