Purification of hemoglobin by displacement chromatography
US5545328A · kind A · utility
25Cited by
12References
12Claims
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Key dates
| Filing date | Mar 20, 1995 |
| Grant date | Aug 13, 1996 |
| Priority date | — |
| Expiry date | Mar 20, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/02
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A preselected hemoglobin species is separated from contaminants having a different acidity from that of the preselected hemoglobin species, by an overload displacement chromatography process. To remove more acidic contaminants, the process is conducted under anion exchange conditions. To remove more basic contaminants, the process is conducted under cation exchange conditions. In either case, the exchange column is overloaded to displace the hemoglobin species therefrom with contaminants having greater affinity for the column, and using the impure hemoglobin solution as the displacer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.