Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5545522A · kind A · utility
235Cited by
2References
14Claims
0Family size
Inventors
Key dates
| Filing date | Oct 5, 1992 |
| Grant date | Aug 13, 1996 |
| Priority date | — |
| Expiry date | Oct 5, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to the use of promoters for ribonucleic acid amplification and other genetic manipulations. Processes are provided wherein complementary deoxyribonucleic acid (cDNA) is synthesized from a ribonucleic acid (RNA) sequence using a complementary primer linked to an RNA polymerase promoter region complement and then anti-sense RNA (aRNA) is transcribed from the cDNA by introducing an RNA polymerase capable of binding to the promoter region. Additional processes using the resulting aRNA are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.