Compositions and methods for chromosome region-specific probes
US5545524A · kind A · utility
34Cited by
0References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 3, 1993 |
| Grant date | Aug 13, 1996 |
| Priority date | — |
| Expiry date | Aug 3, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a rapid reproducible method for generating chromosome region-specific probes for diagnostic and research applications. The method of the present invention comprises microdissecting a chromosome of interest to produce a DNA fragment, treating the dissected DNA fragment with topoisomerase I, amplifying the treated dissected DNA fragment and labelling the amplified DNA. By utilizing the method of the present invention, region-specific probes for fluorescence in situ hybridization (FISH) from a single microdissected chromosome may be generated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.