DNA encoding fused di-alpha globins and production of pseudotetrameric hemoglobin
US5545727A · kind A · utility
24Cited by
30References
40Claims
0Family size
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Key dates
| Filing date | Nov 8, 1991 |
| Grant date | Aug 13, 1996 |
| Priority date | — |
| Expiry date | Nov 8, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/92
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained. DNA encoding alpha globin fusion proteins is provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.