Method for promoting specific alignment of short oligonucleotides on nucleic acids
US5547843A · kind A · utility
26Cited by
3References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 18, 1994 |
| Grant date | Aug 20, 1996 |
| Priority date | — |
| Expiry date | Oct 18, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for promoting specific alignment of short oligonucleotides on a nucleic acid polymer. The nucleic acid polymer is incubated in a solution containing a single-stranded DNA-binding protein and a plurality of oligonucleotides which are perfectly complementary to distinct but adjacent regions of a predetermined contiguous nucleotide sequence in the nucleic acid polymer. The plurality of oligonucleotides anneal to the nucleic acid polymer to form a contiguous region of double stranded nucleic acid. Specific application of the methods disclosed include priming DNA synthesis and template-directed ligation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.